Development and Validation of a HPLC-UV Method for Simultaneous Determination of Lutein and β -Carotene in Food Formulated with Lutein Diester and β-carotene

Lang, Yuting; Liu, Chao; Deng, Cora; Zhang, Yanjun; Chang, Peter; Swanson, Gary

Yuting Lang, Chao Liu, Cora Deng, Yanjun Zhang, Peter Chang, Gary Swanson

Development and Validation of a HPLC-UV Method for Simultaneous Determination of Lutein and β -Carotene in Food Formulated with Lutein Diester and β-carotene

Abstract:

A simple and rapid method has been developed for determination of lutein and β-carotene in food formulated with lutein diester beadlet, microencapsulated β-carotene as ingredients by reverse-phase high performance liquid chromatograph. This method combines extraction and saponification to one step reaction and lack of evaporation. Dimethyl sulfoxide (DMSO) was selected as solvent to release lutein diester, β-carotene from lutein diester beadlet and microencapsulated β-carotene, then add acetone-ethanol-20%KOH/methanol (10:5:4, v/v/v) to saponify lutein diester as well as extract lutein and β-carotene directly at the same time. The prepared sample solution was subjected to a HPLC analysis using Athena C30 column eluting with Methanol / methyl tert-butyl ether (MTBE) mobile phase. The precision, accuracy, linearity, specificity and ruggedness of the developed method were validated following AOAC guidance for single laboratory validation procedure. The recovery of lutein and β-carotene are 94.80%-104.76% and 98.94%-104.69% respectively. The injection repeatability: RSD(r) =1.8% for lutein, RSD(r) =5.5% for β-carotene. This method developed can be used for the quantitation of free lutein and β-carotene in products adding with lutein diester and β-carotene in quality control.

Keywords:
lutein; β -carotene; HPLC; saponification; validation

Issue: 2023, Volume 74, Issue 4

Pages: 27-35

Publication date: 2023/10/1

https://doi.org/10.37358/RC.23.4.8575

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Citation Styles

Cite this article as:
LANG, Y., LIU, C., DENG, C., ZHANG, Y., CHANG, P., SWANSON, G., Development and Validation of a HPLC-UV Method for Simultaneous Determination of Lutein and β -Carotene in Food Formulated with Lutein Diester and β-carotene, Rev. Chim., 74(4), 2023, 27-35.

Vancouver
Lang Y, Liu C, Deng C, Zhang Y, Chang P, Swanson G. Development and Validation of a HPLC-UV Method for Simultaneous Determination of Lutein and β -Carotene in Food Formulated with Lutein Diester and β-carotene. Rev. Chim.[internet]. 2023 Apr;74(4):27-35. Available from: https://doi.org/10.37358/RC.23.4.8575

APA 6th edition
Lang, Y., Liu, C., Deng, C., Zhang, Y., Chang, P. & Swanson, G. (2023). Development and Validation of a HPLC-UV Method for Simultaneous Determination of Lutein and β -Carotene in Food Formulated with Lutein Diester and β-carotene. Revista de Chimie, 74(4), 27-35. https://doi.org/10.37358/RC.23.4.8575

Harvard
Lang, Y., Liu, C., Deng, C., Zhang, Y., Chang, P., Swanson, G. (2023). 'Development and Validation of a HPLC-UV Method for Simultaneous Determination of Lutein and β -Carotene in Food Formulated with Lutein Diester and β-carotene', Revista de Chimie, 74(4), pp. 27-35. https://doi.org/10.37358/RC.23.4.8575

IEEE
Y. Lang, C. Liu, C. Deng, Y. Zhang, P. Chang, G. Swanson, "Development and Validation of a HPLC-UV Method for Simultaneous Determination of Lutein and β -Carotene in Food Formulated with Lutein Diester and β-carotene". Revista de Chimie, vol. 74, no. 4, pp. 27-35, 2023. [online]. https://doi.org/10.37358/RC.23.4.8575

Text
Yuting Lang, Chao Liu, Cora Deng, Yanjun Zhang, Peter Chang, Gary Swanson,
Development and Validation of a HPLC-UV Method for Simultaneous Determination of Lutein and β -Carotene in Food Formulated with Lutein Diester and β-carotene,
Revista de Chimie,
Volume 74, Issue 4,
2023,
Pages 27-35,
ISSN 2668-8212,
https://doi.org/10.37358/RC.23.4.8575.
(https://revistadechimie.ro/Articles.asp?ID=8575)
Keywords: lutein; β -carotene; HPLC; saponification; validation

RIS
TY - JOUR
T1 - Development and Validation of a HPLC-UV Method for Simultaneous Determination of Lutein and β -Carotene in Food Formulated with Lutein Diester and β-carotene
A1 - Lang, Yuting
A2 - Liu, Chao
A3 - Deng, Cora
A4 - Zhang, Yanjun
A5 - Chang, Peter
A6 - Swanson, Gary
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2023
VL - 74
IS - 4
SP - 27
EP - 35
UR - https://doi.org/10.37358/RC.23.4.8575
KW - lutein
KW - β -carotene
KW - HPLC
KW - saponification
KW - validation
ER -

BibTex
@article{RevCh2023P27,
author = {Lang Yuting and Liu Chao and Deng Cora and Zhang Yanjun and Chang Peter and Swanson Gary},
title = {Development and Validation of a HPLC-UV Method for Simultaneous Determination of Lutein and β -Carotene in Food Formulated with Lutein Diester and β-carotene},
journal = {Revista de Chimie},
volume = {74},
number = {4},
pages = {27-35},
year = {2023},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.23.4.8575},
url = {https://revistadechimie.ro/Articles.asp?ID=8575}
}

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